Laboratory of genetic analysis
Infrastructure
NGS sequencer for whole‐genome sequencing
Thermocycler gradient
Vertical electrophoresis with power supply
Horizontal electrophoresis with power supply
Gel documentation system
High-capacity centrifuge
PCR workstation
More:
- Vortex
- Thermo-block
- DNA isolation station
Cooperating Laboratories
- Department/Institute of Plant and Environmental Sciences (FAPZ) – Prof. Ing. PaedDr. Jana Žiarovská, PhD.
- Institute of Botany, Jagiellonian University in Krakow – dr hab. Aneta Słomka prof.
- Institute of Horticulture (FZKI) – Prof. Ing. Miroslava Kačániová, PhD.
- Institute of Biotechnology (FBP) – Ing. Eva Tvrdá, PhD.
- SAV Zvolen
- SAV Mlyňany
Scientific and Research Activities of the Laboratory
- Homogenization of various plant tissues
- Implementation and optimization of isolations of nucleic acids (DNA, RNA, miRNA) and proteins from various types of tissues and plant materials.Verification of the functionality of the isolated nucleic acids using PCR.
- Determination of inter-varietal and interspecies polymorphism of plant samples using marker techniques (RFLP, PBA, iPBS, CDDP, BBAP, PBAP). Study of the population molecular characteristics of plant species.
- Preparation of samples for sequencing (amplification of the selected region and subsequent purification) with a focus on DNA fingerprinting and species differentiation.
- Electrophoretic separation of sample amplicons on agarose (AGE) or polyacrylamide (PAGE) gels and subsequent visualization of the amplified regions using a gel documentation system.
Solved Projects
Current projects
- VEGA 1/0067/24 Timely prevention of bacterial contamination in the cryopreservation process of animal sperm. Project coordinator: Ing. Eva Tvrdá, PhD.
- 09I05-03-V02-00085 Autonomous cultivation system as a potential sustainable source of healthy crops. Project coordinator: doc. Ing. Alena Andrejiová, PhD.
- APVV-20-0058 PThe potential of plant essential oils from aromatic plants for medicinal use and food preservation. Project coordinator: prof. Ing. Miroslava Kačániová, PhD.
Successfully completed projects
- VEGA 1/0163/18 Determination of the pleiotropic effect of endocrine disruptors and biologically active substances in an in vitro cellular model system. Project coordinator: Ing. Zuzana Kňažická, PhD.
- VEGA 2/0025/15 ndophytic microorganisms and their potential role in increasing the tolerance of woody plants to stress. Project coordinator: Ing. Juraj Medo, PhD., Department of Microbiology, FBP SUA in Nitra.
- COST Action FA1402 Improving Allergy Risk Assessment Strategy for new food proteins.
- COST CA 15223 Modifying plants to produce interfering RNA
Publications
Selected publications for the period 2020-2024
ADC Natural Variability of Genomic Sequences of Mal d 1 Allergen in Apples as Revealed by Restriction Profiles and Homolog Polymorphism
Urbanová, Lucia — Bilčíková, Jana — Moravčíková, Dagmar — Žiarovská, Jana. In: Agronomy-Basel. 14, 2056 (2024), s. 9. ISSN 2073-4395.
ADM DNA-Based Variability of Length Polymorphism of Plant Allergens Coding Genes Homologs in Selected Lamiaceae Herbs
Urbanová, Lucia — Farkasová, Silvia — Speváková, Ivana — Kyseľ, Matúš — Šimora, Veronika — Kačániová, Miroslava — Žiarovská, Jana. Beachwood, LIDSEN Publishing Inc. (Beachwood, USA) : (2024).
AAB Charakteristika odrôd viniča hroznorodého pestovaného v oblasti Veľkého Krtíša
Žiarovská, Jana — Fikselová, Martina — Farkasová, Silvia. 1. vyd. Nitra : Slovenská poľnohospodárska univerzita, 2024. 107 s. ISBN 978-80-552-2807-5.
ADN Variability of amplified profiles generated by BBAP in Avena sativa L.
Farkasová, Silvia — Droppa, Michal — Žiarovská, Jana. In: Journal of Microbiology, Biotechnology and Food Sciences. 12, 5 (2023), s. 2023. ISSN 1338-5178.
ADD Utilization of Bet v 1 homologs based amplified profile (BBAP) variability in allergenic plants fingerprinting
Žiarovská, Jana — Urbanová, Lucia. In: Biologia. 77, 2 (2022), s. 517–523. ISSN 0006-3088.
Publications with acknowledgments to VC ABT for the period 2016 and 2017 (recorded in the EPCA database
ADM Central and Eastern European spring pollen allergens and their expression analysis-state of the art / Jana Žiarovská, Lucia Zeleňáková. — ilustr., tab. — AgroBioTech 26220220180. In: Diversity. — ISSN 1424-2818. — vol. 8, no. 4 (2016), s. 1-11.
Effect of DNA extraction method in the Rosa canina L. identification under different processing temperature / [elektronický zdroj] / Jana Žiarovská .. [et al.]. — grafy, ilustr., tab. — Popis urobený 16.5.2017. — Project no. 26220220180. In: Potravinárstvo. — ISSN 1337-0960. — vol. 11, no. 1 (2017), s. 190-196, online. — 10.5219/717.
Content
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