Laboratory of Cell biology and Cytogenetics
Infrastructure
Spectrophotometer – combined spectro-fluoro-luminometer
Spectrophotometer UV-VIS – ultraviolet-visible light spectroscopy
Fluorescence microscope
Incubator with shaker
Sonicator
Laboratory view
Cooperating Laboratories
- Department of Genetics and Breeding Biology, SUA in Nitra: Ing. Alica Navrátilová, PhD.
- Department of Plant Physiology, SUA in Nitra: Ing. Marek Kovár, PhD.
- Comenius University in Bratislava, Department of Genetics
- Slovak Academy of Science, Centre of Biosciences SAS, Institute of Animal Biochemistry and Genetics
- Max F. Perutz Laboratories in Vienna, Austria
Scientific and Research Activities of the Laboratory
- investigation of the role of toxic elements such as Cd, Ni, and Al, or food-born and environmental contaminants on fundamental processes of all living organisms including cell growth, cell morphology, regulation of chromosome segregation during cell cycle
- determination of the antioxidant status of the organism upon toxic element treatment and characterization of the possible positive effect of biologically active substances such as ascorbic acid against toxicity of the particular contaminant
- Determination of the cellular defense mechanisms on molecular basis
Solved Projects
Projects of the laboratory
APVV-22-0294
Funkčná analýza TOR signálnej dráhy v regulácii abiotického stresu v kvasinke Schizosaccharomyces pombe
VEGA 1/0583/23
Odpoveď organizmu na environmentálny stres závislá od signalizácie TORC2 kinázového komplexu
12-GASPU-2021
Zmiernenie toxického účinku akrylamidu pôsobením biologicky aktívnych látok,
Project participation
ITMS2014+ 313011V336 (Drive4SIFood)
dôsledky potravinových kontaminácií na molekulárnej úrovni
ITMS2014+ 313012P694 (Frost)
determinácia toxicity akrylamidu na živé organizmy
ITMS2014+ 313011W580 (Uranos)
analýza antioxidačnej kapacity organizmu ako dôsledok stresových podmienok životného prostredia
APVV-19-0598
testovanie vplyvu extraktu hlivy ustricovej na živé organizmy stresované kontamináciou životného prostredia
Publications
Navrátilová, A., Kovár, M. and Požgajová, M., 2021. Ascorbic acid mitigates cadmium-induced stress, and contributes to ionome stabilization in fission yeast. Environmental Science and Pollution Research, 28(12), pp.15380-15393. https://doi.org/10.1007/s11356-020-11480-x
Požgajová, M., Navrátilová, A., Šebová, E., Kovár, M. and Kačániová, M., 2020. Cadmium-Induced Cell Homeostasis Impairment is Suppressed by the Tor1 Deficiency in Fission Yeast. International journal of molecular sciences, 21(21), p.7847. https://doi.org/10.3390/ijms21217847
Pozgajova, M., Navratilova, A., Arvay, J., Duranova, H. and Trakovicka, A., 2020. Impact of cadmium and nickel on ion homeostasis in the yeast Schizosaccharomyces pombe. Journal of Environmental Science and Health, Part B, 55(2), pp.166-173. https://doi.org/10.1080/03601234.2019.1673613
Pozgajova, M. and Duranova, H., 2021. BIOLUMINOMETRIC ASSAY OF CELL ATP DETECTION UNDER STRESS CONDITIONS AND IN THE ASSESSMENT OF CLEANNESS OF THE FOOD PRODUCING COMPANY. Journal of microbiology, biotechnology and food sciences, 10(5), pp.e2584-e2584. https://doi.org/10.15414/jmbfs.2584
Klongová, L.; Kovár, M.; Navrátilová, A.; Fialkova, V.; Požgajová, M. Cornus mas L. Extract-Mediated Modulations of the Redox State Induce Cytotoxicity in Schizosaccharomyces pombe. Appl. Sci. 2024, 14, 4049. https://doi.org/ 10.3390/app14104049
Požgajová, M.; Klongová, L.; Kovár, M.; Navrátilová, A. 2024. Cell Protection by Oxidative Stress Mitigation Using Substances with Bioactive Properties. In : The Power of Antioxidants – Unleashing Nature’s Defense Against Oxidative Stress (Eds: Novo Barros, A. and Santos Abraão, A.C). doi: 10.5772/intechopen.1004522
Požgajová, M.; Kovár, M.; Navrátilová, A.; Fialková, V.; Chlebová, Z. 2023. Oyster mushroom (Pleurotus ostreatus) ethanolic extract protects yeast cells from hyper-and hypo-glycemic stress. Journal of microbiology, biotechnology and food sciences. 13 (4): e9817. doi: 10.55251/jmbfs.9817
Ďúranová, H.; Šimora, V.; Ďurišová, L.; Olexiková, L.; Kovár, M.; Požgajová, M. 2023. Modifications in ultrastructural characteristics and redox status of plants under environmental stress: A review. Plants. 12 (8): 1666. doi: 10.3390/plants12081666
Navrátilová, A.; Kovár, M.; Kopčeková, J.; Mrázová, J.; Trakovická, A.; Požgajová, M. 2023. Protective effect of Aronia melanocarpa juice against acrylamide-induced cellular toxicity. Journal of Environmental Science and Health, Part B. 58 (2): 139-149. doi: 10.1080/03601234.2023.2172287
Požgajová, M.; Navrátilová, A.; Kovár, M. 2022. Curative Potential of Substances with Bioactive Properties to Alleviate Cd Toxicity: A Review. International Journal of Environmental Research and Public Health. 19 (19): 12380. doi: 10.3390/ijerph191912380
Kovár, M.; Navrátilová, A.; Kolláthová, R.; Trakovická, A.; Požgajová, M. 2022. Acrylamide-Derived Ionome, Metabolic, and Cell Cycle Alterations Are Alleviated by Ascorbic Acid in the Fission Yeast. Molecules. 27 (13): 4307. doi: 10.3390/molecules27134307
Navrátilová, A.; Kovár, M.; Trakovická, A.; Požgajová, M. 2021. Cell homeostasis alterations induced by environmental stress. Acta Fytotechnica et Zootechnica. 24 (3): 226-232. doi: 0.15414/afz.2021.24.03.226-232
Navratilova, A.; Kovar, M.; Trakovicka, A; Pozgajova, M. 2021. Nickel induced cell impairments are negatively regulated by the Tor1 kinase in Schizosaccharomyces pombe. World Journal of Microbiology and Biotechnology. 37: 165. doi: 10.1007/s11274-021-03130-2
Kovár, M.; Navrátilová, A.; Trakovická, A.; Požgajová, M. 2021. Ascorbic acid supplementation suppresses cadmium-derived alterations in the fission yeast Schizosaccharomyces pombe. Slovak Journal of Food Sciences. 15: 423-432. doi: 10.5219/1618
Content
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