Laboratory of Agrobiodiversity and Genetic Technologies
Infrastructure
Thermocycler for direct PCR
Spectrophotometer
Real-Time Thermocycler
PCR workstation
Microarray system
Thermo-block
More:
- Thermocycler gradient
- Gradient real-time PCR
- Hybridization oven
Cooperating Laboratories
- Department/Institute of Plant and Environmental Sciences (FAPZ) – Prof. Ing. PaedDr. Jana Žiarovská, PhD.
- Institute of Botany, Jagiellonian University in Krakow – dr hab. Aneta Słomka prof.
- Institute of Horticulture (FZKI) – Prof. Ing. Miroslava Kačániová, PhD.
- Institute of Biotechnology (FBP) – Ing. Eva Tvrdá, PhD.
- SAV Zvolen
- SAV Mlyňany
- Faculty of Tropical AgriScience, CULS Prague
Scientific and Research Activities of the Laboratory
- Design of primers, creation and optimization of PCR methodologies.
- Synthesis of cDNA molecules.
- Determination of gene expression levels for mapping the influence of stress factors in plants.
- Detection of plant pathogens using real-time PCR.
- Analysis of allergen gene expression, focusing on PR-10 proteins.
- Identification of miRNA stress biomarkers.
- Analysis of cell lines using rt-PCR, monitoring changes in the expression of genes involved in inflammatory processes, apoptosis-related genes, and oxidative stress.
Solved Projects
Current projects
- SK-PL-23-0063 Risks of toxic heavy metals to selected crop plants classified as superfood of the 21st century. (Project coordinator: prof. PaedDr. Ing. Jana Žiarovská, PhD.)
- VEGA Plasticity of the mallow in response to heavy metals: a multilevel analysis from eco-physiological to molecular aspects. Project coordinator: prof. PaedDr. Ing. Jana Žiarovská, PhD.
- APVV-20-0058 The potential of plant essential oils from aromatic plants for medicinal use and food preservation. Project coordinator: prof. Ing. Miroslava Kačániová, PhD.
- APVV-22-0348 Potential anticancer effect of MCT coconut oil determined by addition of plant extracts from selected Capsicum spp. fruits (Project coordinator: RNDr. Hana Ďúranová, PhD.)
- GA SPU MicroRNA – potential predictor of the quality of cryopreserved sperm.
Project coordinator: Ing. Michal Ďuračka, PhD. - VEGA 1/0067/24 Timely prevention of bacterial contamination in the cryopreservation process of animal sperm. Project coordinator: Ing. Eva Tvrdá, PhD.
- 09I05-03-V02-00085 Autonomous cultivation system as a potential sustainable source of healthy crops. Project coordinator: doc. Ing. Alena Andrejiová, PhD.
Successfully completed projects
- VEGA 1/0083/16 Study of population and reproductive parameters of selected plant taxa in the agricultural landscape of Slovakia. Project coordinator: doc. Ing. Pavol Eliáš, PhD., Department of Botany, FAFR SUA in Nitra.
- VEGA 1/0411/17 Determination of the effect of biologically active substances in the wine production process on microbial and ovarian cells. Project coordinator: prof. Ing. Miroslava Kačániová, PhD., Department of Microbiology, FBFS SUA in Nitra.
- VEGA 2/0025/15 Endophytic microorganisms and their potential role in increasing the tolerance of woody plants to stress. Project coordinator: Ing. Juraj Medo, PhD., Department of Microbiology, FBFS SUA in Nitra.
- COST Action FA1402 Improving Allergy Risk Assessment Strategy for new food proteins.
- COST CA 15223 Modifying plants to produce interfering RNA.
- APVV-Dunajská stratégia DS-2016-0051 Genomics, transcriptomics, digestomics, and a mouse sensitization model for allergenic lipid transfer proteins.
- APVV-15-0156 Genomic selection of cereals for drought resistance. Project coordinator: doc. RNDr. Miroslav Švec, CSc., Comenius University in Bratislava.
- VEGA 2/0041/16 Molecular methods in the breeding of naturally gluten-free amaranth. Project coordinator: prof. RNDr. Zdenka Gálová, PhD.
Publications
Selected publications for the period 2024-2025
ADC Chemical-analytical characterization and Pun1 gene expression variability analysis of ten selected Capsicum varieties
Kováčiková, Eva — Urbanová, Lucia — Kuželová, Lenka — Lakatošová, Jana — Jančo, Ivona – Árvay, Július – Žiarovská, Jana – Ďúranová, Hana. In: Food Bioscience. 65, 106038 (2025), s. 2025. ISSN 2212-4292.
AFH Gene and protein expression patterns of Bax and Bcl-2 genes in cryopreserved bovine spermatozoa. Benko, Filip – Fialková, Veronika – Žiarovská, Jana – Ďuračka, Michal – Tvrdá, Eva. In: CHRENEK, Peter. Animal biotechnology 2024. Nitra: Slovak University of Agriculture, 2024, s. 59. ISBN 978-80-552-2715-3.
ADF Sequence based prediction of 26S rRNA in medicinal plants for qRT-PCR purposes. Žiarovská, Jana – Urbanová, Lucia – Kučerová, Lenka – Kováčik, Adam – Kačániová, Miroslava. In: Journal of Horticulture, Forestry and Biotechnology. 28, 1 (2024), s. 101–107. ISSN 2066-1797.
Publications with acknowledgments to VC ABT for the period 2016 and 2017 (recorded in the EPCA database
ADC Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods / Jana Žiarovská .. [et al.]. — ilustr. — projekt č. 20165007, projekt č. 26220220180: „AgroBioTech“. In: Czech journal of food sciences. — ISSN 1212-1800. — vol. 34, no. 6 (2016), s. 495-502.
ADE Pidchody do kiľkisnogo vyznačennja 5-gidroksymetylfurfurolu v likarskych zasobach ta charčovych produktach / N. I. Gudz .. [et al.]. — Projekt no. ITMS 26110230085, ITMS 26220220180. In: Farmakom. — ISSN 2414-9195. — no.3 (2016), s. 41-45.
ADM Direct PCR as the platform of Hedera helix, L. genotypying without the extraction of DNA [elektronický zdroj] = Priama PCR bez použitia izolácie DNA ako platforma genotypovania Hedera helix, L. / Danka Bošeľová, Jana Žiarovská. — ilustr., tab. — Názov z titulnej obrazovky (stav zo dňa 02.02.2017). — AgroBioTech 26220220180. In: Journal of Central European Agriculture. — ISSN 1332-9049. — vol. 17, no. 4 (2016), online. — 10.5513/JCEA01/17.4.1795.
ADM Central and Eastern European spring pollen allergens and their expression analysis-state of the art / Jana Žiarovská, Lucia Zeleňáková. — ilustr., tab. — AgroBioTech 26220220180. In: Diversity. — ISSN 1424-2818. — vol. 8, no. 4 (2016), s. 1-11.
ADN Effect of DNA extraction method in the Rosa canina L. identification under different processing temperature / [elektronický zdroj] / Jana Žiarovská .. [et al.]. — grafy, ilustr., tab. — Popis urobený 16.5.2017. — Project no. 26220220180. In: Potravinárstvo. — ISSN 1337-0960. — vol. 11, no. 1 (2017), s. 190-196, online. — 10.5219/717.
AED Soderžanije organičeskich kislot v plodach Ziziphus jujuba Mill. = The organic acids content in the fruit of Ziziphus jujuba Mill. / M. Ju. Karnatovskaja .. [et al.]. — grafy, ilustr. — Projekty TRIVE ITMS 26110230085, ITEBIO ITMS 26220220115, AgroBioTech ITMS 26220220180.In: Biodiversity after the Chernobyl accident. — 1st ed.. — 240 s.. — 978-80-552-1527-3 (brož.). — Nitra : Slovak University of Agriculture, 2016. — s. 103-106.
AED Biochimičeskije osobennosti i lekarstvennyje svojstva kizila nastojaščego (Cornus mas L.) = Biochemical features and medical properties of cornelian cherry (Cornus mas L.) / Svitlana Klymenko .. [et al.]. — grafy, ilustr., tab. — Projekty TRIVE ITMS 26110230085, ITEBIO ITMS 26220220115, AgroBioTech ITMS 26220220180. In: Biodiversity after the Chernobyl accident. — 1st ed.. — 240 s.. — 978-80-552-1527-3 (brož.). — Nitra : Slovak University of Agriculture, 2016. — S. 111-118.
AED Jakicť i bezpeka medu z riznych regioniv Ukrajiny = Quality and safety of honey from different regions of Ukraine / L. O. Adamčuk .. [et al.]. — ilustr., tab. — Projekt AgroBioTech. In: Biodiversity after the Chernobyl accident. — 1st ed.. — 240 s.. — 978-80-552-1527-3 (brož.). — Nitra : Slovak University of Agriculture, 2016. — s. 10-14.
AFC Obsah polyfenolov a atioxidačnej aktivity tokajských esencií / Zuzana Eftimová, Jarmila Eftimová, Jan Brindza. — ilustr., tab. — ITMS 26220220180 AgroBioTech. In: Sučasni aspekty zberežennja zdorovja ljudyny. — 1. vypusk. — 389 s.. — 978-617-7333-40-0 (brož.) Mižnarodna miždisciplinarna naukovo-praktyčna konferencija. — Užhorod : Užgorodskij nacionaľnij universitet, 2017. — s. 78-80.
AFD Genomická variabilita včlenení retrotranspozónov druhu Hedera helix L. = Genomic variability of retrotransposon insertions in Hedera helix L. / Danka Bošeľová, Jana Žiarovská. — ilustr., tab. — AgroBioTech ITMS 26220220180. In: Dendrologické dni v Arboróte Mlyňany SAV 2016. — 1. vyd.. — 255 s.. — 978-80-89408-26-9 (brož.) Dendrologické dni. — Vieska nad Žitavou : Arborétum Mlyňany SAV, 2016. — s. 35-40.
AFH Flax dLUTE transposon as DNA marker / Jana Žiarovská, Veronika Lancíková, Lucia Zeleňáková, Milan Bežo. — AgroBioTech 26220220180, APVV 0740-11. In: Book of abstracts and posters of the 11th international scientific conference Biotechnology and quality of raw materials and foodstuffs. — 1st ed.. — 136 s.. — 978-80-552-1452-8 (brož.) Biotechnology and quality of raw materials and foodstuffs. — Nitra : Slovak University of Agriculture, 2016. — s. 82
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